Isolation and Determination of Lactoferrin in Human Saliva

Authors

  • S. Skaličková Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University, Brno
  • O. Zítka Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University, Brno
  • S. Křížková Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University, Brno
  • M. Vlková Department of Clinical Immunology and Allergology, University Hospital, Brno
  • J. Sochor Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University, Brno | Central European Institute of Technology, University of Technology, Brno
  • V. Adam Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University, Brno | Central European Institute of Technology, University of Technology, Brno
  • R. Kizek Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University, Brno | Central European Institute of Technology, University of Technology, Brno

Keywords:

lactoferrin, saliva, ion-exchange liquid chromatography, photometric detection

Abstract

Lactoferrin, a globular glycoprotein, is an important component of saliva. It shows an antibacterial, anticancerogenic and anti-inflammatory activity. The aim of this study was to develop a method of isolation of lactoferrin from human saliva using ion exchange chromatography in a monolithic column and spectrometric detection with Pyrogallol Red by the Bradford and biuret methods. The calibration curve for lactoferrin was linear in the range 0.06–62.5 μg ml–1, limit of detection 0.01 μg ml–1. The lactoferin concentration in saliva of healthy subjects was 42±4 μg mg–1. Patient with celiac disease showed 2.5×times higher concentration of lactoferrin compared with healthy subjects.

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Published

2014-01-15

How to Cite

Skaličková, S., Zítka, O., Křížková, S., Vlková, M., Sochor, J., Adam, V., & Kizek, R. (2014). Isolation and Determination of Lactoferrin in Human Saliva. Chemické Listy, 108(1), 56–63. Retrieved from http://www-.chemicke-listy.cz/ojs3/index.php/chemicke-listy/article/view/573

Issue

Vol. 108 No. 1 (2014)

Section

Articles

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